KIP publications

year 2016
author(s) Katharina Haase, Niels Kröger-Lui, Annemarie Pucci, Arthur Schönhals and Wolfgang Petrich
title Real-time mid-infrared imaging of living microorganisms
KIP-Nummer HD-KIP 15-88
KIP-Gruppe(n) F6,F23
document type Paper
source Journal of Biophotonics, 9 (2016) 61-66
doi 10.1002/jbio.201500264
Abstract (en)

The speed and efficiency of quantum cascade laser-based mid-infrared microspectroscopy are demonstrated using two different model organisms as examples. For the slowly moving Amoeba proteus, a quantum cascade laser is tuned over the wavelength range of 7.6 µm to 8.6 µm (wavenumbers 1320 cm–1 and 1160 cm–1, respectively). The recording of a hyperspectral image takes 11.3 s whereby an average signal-to-noise ratio of 29 is achieved. The limits of time resolution are tested by imaging the fast moving Caenorhabditis elegans at a discrete wavenumber of 1265 cm–1. Mid-infrared imaging is performed with the 640 × 480 pixel video graphics array (VGA) standard and at a full-frame time resolution of 0.02 s (i.e. well above the most common frame rate standards). An average signal-to-noise ratio of 16 is obtained. To the best of our knowledge, these findings constitute the first mid-infrared imaging of living organisms at VGA standard and video frame rate.

  author   = {Haase, Katharina and Kröger-Lui, Niels and Pucci, Annemarie and Schönhals, Arthur and Petrich, Wolfgang},
  title    = {Real-time mid-infrared imaging of living microorganisms},
  journal  = {Journal of Biophotonics},
  year     = {2016},
  volume   = {9},
  pages    = {61–66},
  doi      = {10.1002/jbio.201500264}
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