Jahr 2014
Autor(en) Niels Kröger, Alexander Egl, Maria Engel, Norbert Gretz, Katharina Haase, Iris Herpich, Bettina Kränzlin, Sabine Neudecker, Annemarie Pucci, Arthur Schönhals, Jochen Vogt, Wolfgang Petrich
Titel Quantum cascade laser-based hyperspectral imaging of biological tissue
KIP-Nummer HD-KIP 14-41
KIP-Gruppe(n) F6,F23
Dokumentart Paper
Quelle Journal of Biomedical Optics 19 (2014) 111607
doi 10.1117/1.JBO.19.11.111607
Abstract (en)

The spectroscopy of analyte-specific molecular vibrations in tissue thin sections has opened up a path toward histopathology without the need for tissue staining. However, biomedical vibrational imaging has not yet advanced from academic research to routine histopathology due to long acquisition times for the microscopic hyperspectral images and/or cost and availability of the necessary equipment. Here we show that the combination of a fast-tuning quantum cascade laser with a microbolometer array detector allows for a rapid image acquisition and bares the potential for substantial cost reduction. A 3.1×2.8  mm2 unstained thin section of mouse jejunum has been imaged in the 9.2 to 9.7 μm wavelength range (spectral resolution ∼1  cm−1) within 5 min with diffraction limited spatial resolution. The comparison of this hyperspectral imaging approach with standard Fourier transform infrared imaging or mapping of the identical sample shows a reduction in acquisition time per wavenumber interval and image area by more than one or three orders of magnitude, respectively.

  author   = {Kr\"oger, Niels and Egl, Alexander and Engel, Maria and Gretz, Norbert and Haase, Katharina and Herpich, Iris and Kr\"anzlin, Bettina and Neudecker, Sabine and Pucci, Annemarie and Sch\"onhals, Arthur and Vogt, Jochen and Petrich, Wolfgang},
  title    = {Quantum cascade laser-based hyperspectral imaging of biological tissue},
  journal  = {Journal of Biomedical Optics},
  year     = {2014},
  volume   = {19},
  number   = {11},
  pages    = {111607},
  doi      = {10.1117/1.JBO.19.11.111607}
URL doi:10.1117/1.JBO.19.11.111607
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