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We have examined transcription loops on
lampbrush chromosomes of the newt Notophthalmus
by superresolution microscopy. Because of the favorable,
essentially two-dimensional morphology of these
loops, an average optical resolution in the x–y plane of
about 50 nm was achieved. We analyzed the distribution
of the multifunctional RNA-binding protein
CELF1 on specific loops. CELF1 distribution is consistent
with a model in which individual transcripts are
tightly folded and hence closely packed against the
loop axis.