KIP publications

Localisation microscopy methods allow to realize a light optical resolution far beyond the Abbe–Rayleigh
limit of about 200nm laterally and 600nm axially. So far, this progress was achieved using labelling with
appropriate fluorochromes and fluorescent proteins. Here, we describe for the first time that optical resolution
of cellular structures in the /10 range (∼50 nm) can be achieved even in label-free cells. This was
obtained using Spectral Precision Distance/Position Determination Microscopy (SPDM), a method based
on the general principles of localisation microscopy. Besides a substantial resolution improvement of
autofluorescent structures, SPDM revealed cellular objects which are not detectable under conventional
fluorescence imaging conditions.