KIP-Veröffentlichungen

Jahr 2019
Autor(en) Rostyslav Semenyshyn, Mario Hentschel, Christian Huck, Jochen Vogt, Felix Weiher, Harald Giessen, and Frank Neubrech
Titel Resonant Plasmonic Nanoslits Enable in Vitro Observation of Single-Monolayer Collagen-Peptide Dynamics
KIP-Nummer HD-KIP 19-44
KIP-Gruppe(n) F6,F28
Dokumentart Paper
Quelle ACS Sens. 2019, 4, 8, 1966-1972
doi 10.1021/acssensors.9b00377
Abstract (en)

Proteins perform a variety of essential functions in living cells and thus are of critical interest for drug delivery as well as disease biomarkers. The different functions are derived from a hugely diverse set of structures, fueling interest in their conformational states. Surface-enhanced infrared absorption spectroscopy has been utilized to detect and discriminate protein monomers. As an important step forward, we are investigating collagen peptides consisting of  a  triple helix. While they constitute the main structural building blocks in many complex proteins, they are also a perfect model system for the complex proteins relevant in biological systems. Their complex spectroscopic information as well as the overall small size present a significant challenge for their detection and discrimination. Using resonant plasmonic nanoslits, which are known to show larger specificity compared to nanoantennas, we overcome this challenge. We perform in vitro surface-enhanced absorption spectroscopy studies and track the conformational changes of these collagen peptides under two different external stimuli, which are temperature and chemical surroundings. Modeling the coupling between the amide I vibrational modes and the plasmonic resonance, we can extract the conformational state of the collages and thus monitor the folding and unfolding dynamics of even a single monolayer. This leads to new prospects in studies of single layers of proteins and their folding behavior in minute amounts in a living environment.

bibtex
@article{Semenyshyn19,
  author   = {Semenyshyn, Rostyslav and Hentschel, Mario and Huck, Christian and Vogt, Jochen and Weiher, Felix and Giessen, Harald and Neubrech, Frank},
  title    = {Resonant Plasmonic Nanoslits Enable in Vitro Observation of Single-Monolayer Collagen-Peptide Dynamics},
  journal  = {ACS Sensors},
  year     = {2019},
  volume   = {4},
  number   = {8},
  pages    = {1966-1972},
  note     = {},
  doi      = {10.1021/acssensors.9b00377},
  url      = {https://doi.org/10.1021/acssensors.9b00377}
}
URL doi:10.1021/acssensors.9b00377
KIP - Bibliothek
Im Neuenheimer Feld 227
Raum 3.402
69120 Heidelberg