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Research


The development of novel nanoscopy methods for the analysis of biological systems in their natural environment is a major question of present biological and biophysical research. Hence, novel light-optical methods are required, allowing to study such biological structures also within living cells, even when their dimension is far below the conventional resolution limit of some hundreds of nanometers. In our research group, two new and complementary methods have been developed: 1. Spectral Precision Distance Microscopy(SPDM) and Single Molecule Localization Microscopy(SMLM) with standard fluorophors, using one laser frequency for both photoswitching and readout. Presently,such procedures allow to analyze biological nanostructures down to a resolution in the few tens of Nanometer range. 2. Structured Illumination (SI) Microscopy. With SI microscopy, sizes of fluorescent objects as low as a few tens of nanometers can be determined with high precision. Laterally structured illumination (SIM) is presently the only linear super-Resolution microscopy technique, allowing Operation at lowest Illumination for in-vivo studies. The structural data obtained by these light optical methods have to be interpreted in the framework of theoretical modelling of the relevant structures. For this, it is necessary to quantify these models and to derive by means of scientific computing consequences which are subject to experimental verification. Here, numerical simulations are used. As an example, a dynamic model of the genomic structure of the human nucleus has been developed, which provides numerous predictions with respect to the 4D geometry, the thermal induced mobility, and the impact of radiation. Ways to achieve these aims is by a combination of the methods of biophotonics, biophysics, biocomputing and information processing